Phytochemical and GC-MS analysis of bioactive compounds of Sphaeranthus amaranthoides Burm

Abstract Objective To isolate and analyze the phytochemical constituents of Sphaeranthus amaranthoides using GC–MS. Method Preliminary phytochemical screening of the extract was carried out according to the standard method described by Brindha et al. GC–MS analysis was performed on the methanolic extract of S. amaranthoides to find out the chemical constituents. Results Phytochemical screening revealed the presence of steroids, alkaloids, sugars, phenolics, flavonoids, saponins, tannins and amino acids with mottled degree. GC–MS results revealed the presence of 23 different phytocompounds viz., 2-Propenoic acid, 2-methyl-, 2-[[2,3,3a,4,7,7a(or 3a,4,5,6,7,7a)-hexahydro-4,7-methano-1H-indenyl]oxy] ethyl ester (32.73%), Methanone, (1-hydroxycyclohexyl)phenyl – (13.71%), Methyl 2-bromomethyl-10-tetrahydropyranyloxy-2-decenoate – (7.84%),4,7-Methano-1H-indene,3a,4,5,6,7,7a-hexahydro-5-(2-propenyloxy) – (6.27%), Primidone – (4.50%), 2-Propenoic acid, 1,7,7-trimethylbicyclo[2.2.1]hept-2-yl ester, exo – (3.78%) and normorphine, bis(o-trimethylsilyl) – (3.65%) etc. Conclusion The presence of various bioactive compounds confirms the application of S. amaranthoides for various diseases by traditional practitioners. However, isolation of individual phytochemical constituents may proceed to find a novel drug.