Evaluation of vitrified goat ovarian tissue after autotransplantation

Transplantation of cryopreserved ovarian tissue before chemotherapy is a promising assisted reproductive technology (TRA) to preserve fertility for prepubertal girls or adult women with cancer. Therefore, this technology can also be applied to preserve the reproductive function in animal’s farm. In this way. The current study was designed to compare fresh and vitrified-warmed goat ovarian tissue after autotransplantation. Eleven adult goats (n =11) were completely ovariectomized after premedication and anesthesia. After ovariectomy, each ovary pair was fragmented (5x5x1mm3 ) and distributed as follow: seven fresh ovarian fragments were directly autotransplanted in the left side of the peritoneum and three fresh fragments fixed in paraformaldehyde 4% for classical histology and named as Fresh Control. Simultaneously, ten fragments were vitrified for one week. After warming, the fragments were autotransplanted to the right side of the peritoneum after a second surgery. The remaining vitrified/warmed fragments (3 fragments) were then fixed in paraformaldehyde 4% for classical histology and named as Vitrified Control. After 90 days of autotransplantation, three antral follicles (two in fresh graft and one in vitrified-warmed graft) were observed on the surface of the grafts. Moreover, one oocyte was punctured from an antral follicle of a fresh fragment. The percentage of normal preantral follicles reduced (P 0.05) of normal follicles was found between fresh control, fresh transplant, and vitrified transplant groups. Moreover, the percentage of developing follicles was similar between fresh graft and vitrified graft. Fresh graft and vitrified graft had a similar odds ratio (P = 0.17) to presence of normal preantral follicles. Thus, these results show that transplantation of vitrified goat ovarian tissue is able to promote development of preantral follicles until antral stage. (CEUA-UECE/protocol#2917497/2015).