Purification of a β-glucosidase from Helix snailase.

The β-glucosidase was purified from Helix snailase through a combination of DEAE stepwise chromatography and DEAE gradient chromatography with gel filtration chromatography.The β-glucosidase was purified to apparent homogeneity on SDS-PAGE.And it was estimated that the purified hydrolase was consisted of four identical subunits with a molecular mass of 110~115 kD by SDS-PAGE and gel filtration chromatography.The Km and Vmax values for β-D-glucopyranoside(pNPG) were calculated to be 0.182 mmol/L and 0.189 units/mg of protein respectively.