Knockout of ptsG Gene in Escherichia coli and Its Influence on Production of L-phenylalanine

L-Phenylalanines are important food and pharmaceutical intermediates.The PTS system is very important to production of L-phenylalanine in culture of Escherichia coli.The ptsG gene for glucose-specific transporter enzyme ⅡCBGlc of the phosphotransferase system was knocked-out so as to reduce the accumulation of acetic acid in culture of Escherichia coli.Knockout of the ptsG gene in Escherichia coli was done by Red recombinant system.The ptsG gene was disrupted to construct the mutant called MD-ptsG-.The MD-ptsG-strain was incubated in the media using glucose as the sole carbon source and the culture density was 3.5 times of the control,L-phe yield increased by 12%.