Multimodal fiber-probe spectroscopy as a clinical tool for diagnosing and classifying biological tissues
2017
An optical fiber probe for multimodal spectroscopy was designed, developed and used
for tissue diagnostics. The probe, based on a fiber bundle with optical fibers of various size and
properties, allows performing spectroscopic measurements with different techniques, including
fluorescence, Raman, and diffuse reflectance, using the same probe. Two visible laser diodes were
used for fluorescence spectroscopy, a laser diode emitting in the NIR was used for Raman
spectroscopy, and a fiber-coupled halogen lamp for diffuse reflectance. The developed probe was
successfully employed for diagnostic purposes on various tissues, including brain and bladder. In
particular, the device allowed discriminating healthy tissue from both tumor and dysplastic tissue
as well as to perform tumor grading. The diagnostic capabilities of the method, determined using a
cross-validation method with a leave-one-out approach, demonstrated high sensitivity and
specificity for all the examined samples, as well as a good agreement with histopathological
examination performed on the same samples. The obtained results demonstrated that the
multimodal approach is crucial for improving diagnostic capabilities with respect to what can be
obtained from individual techniques. The experimental setup presented here can improve
diagnostic capabilities on a broad range of tissues and has the potential of being used clinically for
guiding surgical resection in the near future.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
9
References
0
Citations
NaN
KQI