Antioxidative peptides of hydrolysate prepared from fish skin gelatin using ginger protease activate antioxidant response element-mediated gene transcription in IPEC-J2 cells

Abstract Excess production of reactive oxygen species (ROS) causes oxidative stress, which is associated with oxidative damage and a number of human chronic diseases. Here, novel antioxidant hydrolysates were obtained from fish skin, pig skin, pig bone and bovine skin gelatins using ginger protease, and were compared with those produced using pepsin-pancreatin. Ginger protease could hydrolyze the gelatin from fish skin to peptides with low average molecular weights ( 2 O 2 -induced intracellular ROS production. Overall, our results demonstrate that FSGH prepared using ginger protease could serve as a source of peptides with high antioxidative activities.