The Detection of Peroxynitrite Using 19F Magnetic Resonance Probes

The following report presents the work done by Kevin Bruemmer for his Engaged Learning project. The 19F magnetic resonance probes 5-fluoroisatin and 6-fluoroisatin were reacted with ONOO− to yield anthranilic acid derivatives through a novel oxidative decarbonyaltion reaction. Utilizing 19F NMR spectroscopy techniques, the presence of ONOO− can be determined through a chemical shift switching signal. 5-fluoroisatin was subsequently used to detect ONOO− in living lung epithelial cells. Kevin Bruemmer Engaged Learning Final Report