HPLC-ELSD法测定酵母浸膏中游离L-精氨酸

High performance liquid chromatograpHy(HPLC)coupled with evaporative light scattering detector(ELSD-2000)was used for the determination of L-arginine in yeast extract without derivatization. A Alltima C18(250 mm ×4.6 mm i.d., 5 μm) was used with the mobile pHase of 0.2% trifluoroacetic acid water solution containing 5.0 mmol/L heptafluorobutyric acid and acetonitrile(the ratio of volume was 93.3 to 6.7).The flow rate was at 0.6 mL/min. The drift tube temperature of ELSD was set at 110 ℃,and with the nitrogen flow rate of 2.8 L/min. The calibration curves were showed good linearity(r^2=0.9995) within the concentration range of 20.0~500 g/L for L-arginine. The average recoveries were ranged from 97.57% to 103.5% and the standard deviation was less than 2.39%. The method was evaluated as rapid, accurate and simple. The validated method is successfully applied to quantify L-arginine in yeast extract which provides a new basis of overall assessment on quality of other organic nitrogen source and determination of L-arginine in fermentation broth.