Detection of urinary antigens of Legionella pneumophila serogroup 12 by broad-spectrum enzyme-linked immunosorbent assay.

Abstract The first Canadian case of Legionella pneumophila serogroup 12 infection is reported. The initial diagnosis was made with a urine specimen by a broad-spectrum enzyme-linked immunosorbent assay; further serogrouping was done by indirect fluorescent-antibody and tube agglutination tests. This case emphasizes the importance of multivalent urinary antigen detection for the diagnosis of legionellosis.