Shotgun Proteomics of non-depleted and depleted hemolymph of human Schistosomiasis snail vector; Biomphalaria glabrata, revealed vital immune related proteins

Background: Biomphalaria glabrata is an important host in the transmission of human schistosomiasis in the Caribbean and South America. There are serious setbacks in the current control measures, hence need to explore alternatives. Objective: To unveil Biomphalaria glabrata hemolymph proteome for possible detailed knowledge of its immunity in host-pathogen relationship. Methods: In this study we performed a shotgun proteomic and bioinformatic analyses of the non-depleted and depleted [0.5 and 0.75% Trifluoroacetic acid (TFA) depletion] hemolymph of B. glabrata (LE strain). Results: Our analysis was able to mine a total of 148 proteins from the hemolymph. The highest number of protein (148) was obtained from the 0.5% TFA-depleted sample, followed by 62 proteins from the 0.75% TFA-depleted sample and only 59 from non-depleted hemolymph. Conclusion: Most of these proteins are novel and some of them may perform an important role in the immunity of this snail against invading pathogens.