DETECTION OF BACILLUS CEREUS CONTAINING VOITOXIN IN FOOD BY LOOP-MEDIATED ISOTHERMAL AMPLIFICATION METHOD

Bacillus cereus strains containing voitoxin cereulide are pathogenic bacteria. The cereulide is produced by B.cereus strains containing plasmid pCER270.To establish a specific loop-mediated isothermal amplification (LAMP) method detecting pathogenic B. cereus, LAMP primer sets were designed based on 16SrDNA and pCER270plasmidcesAsequence.TheLAMPreactionwascarriedoutunderoptimal conditionswith2 mMof Mg 2+ at65Cfor1 h.Specificityof LAMPprimerswerevalidated by assaying 19 B. cereus strains with and without pCER270 plasmid and 42 non-B.cereusspecies.Detectionlimitsof theLAMPassaywere1 cfu/mLof B.cereus without pCER270 plasmid and 11 cfu/mL of pathogenic B. cereus with pCER270 plasmid.Furthermore,liquidmilksamplesweredetectedbytheLAMPmethod.The resultsshowedthatLAMPmethodisareliablemethodusedforrapiddetectionofB. cereusand its pathogenic strains of milk and other food stuff.