Lipid peroxidation in patients with acute and chronic pancreatitis

OBJECTIVE: To assess the initial levels of lipid peroxidation (LPO) products in LDL and to study changes in oxidative resistance (OR) of precipitated LDL in acute and chronic pancreatitis patients (OP and CP). METHODS: were examined 39 CP patients and 37 OP patients, and the control group - 7 persons without pancreatic diseases and diabetes mellitus (DM). All CP patients were examined in the acute stage, OP patients - in the first 5 days after admission to hospital. Determination of LPO products in LDL and OR of LDL to Cu2+ - induced oxidation were carried out its own method (Ragino, 1998). RESULTS: In CP patients the initial level of LPO products in LDL was higher (8.9 +/- 1.5 nmol MDA/mg LDL protein) than in those in control group (5.3 +/- 0.8) and in OP patients (5.0 +/- 0.5, p<0.05), and the OR of LDL is significantly lower than those in the control group. In AP and CP patients with permanent pain syndrome in the left hypochondrium initial level of LPO products in LDL is much higher (7.0 +/- 0.6, and 5.0 +/- 0.6, p = 0,016), and OR of LDL after 0, 5, 1 and 2 h incubation with Cu2+ (2 h: 31.7 +/- 1.8 and 23.2 +/- 1.9, p = 0.002) - much lower compared with AP and CP patients without pain. Only CP patients combined with DM type 2 were defined reducing the OR of LDL after 0.5, 1 and 2 h incubation with Cu2+. CONCLUSION: The activity of LPO in the blood and LDL were affected in AP and CP patients.