Production of Somatic Hybrid between Black Mustard (Brassica nigra KOCH.;BB) and Hakuran (B. napus L.;AACC)

Mesophyll protoplasts of black mustard and Hakuran were fused by the PEG-high pH ?? igh Ca method. Out of six plants regenerated, one showed hybrid morphology. The hybridity was ascertained by the isozyme pattern of acid phosphatase and chromosome number. This hybrid was estimated to be a trigenomic hexaploid having A. B and C genomes, and showed similar male sterility to Hakuran.