Determination of Amylose and Amylopectin Content in Fresh Corn by Dual-wavelength Spectrophotometry

以碘为显色剂,应用双波长分光光度法测定了鲜食玉米中直链淀粉和支链淀粉的含量.根据碘-直链淀粉和碘-支链淀粉复合物的吸收光谱,选择直链淀粉的测定波长为629 nm和463 nm,支链淀粉的测定波长为553 nm和738 nm,并依据回归方程求出直链淀粉和支链淀粉的含量.直链淀粉的含量在10～60 mg/L、支链淀粉在60～200 mg/L范围内符合比耳定律.用双波长法测定不同品种鲜食玉米中直链淀粉和...