Environmental Risk Indication for the Wastewater of the Bio-Pharmaceutical Industry by Microbial Fitting Function

Trying to work out a fast, simple and convenient assessment method for the wastewater of bio-pharmaceutical industry, microcosm, dose-effective relation analysis and fitting function analysis were performed, according to the better reactivity of microbial in pharmaceutical environmental risk assessment, to select representative microbial indicator on the basis of coefficient of fitting function. Among 4 candidates, a significant negative co-relation (r=-0.981, P=0.003) among the mass growths (OD600) of Aerobacter cloacae and the concentrations of artificial composed water was seen with a coefficient (R2) of 0.9626. So the aerobacter cloacae was selected as an indicator, and its equation of fitting function was: y=-0.0634x+0.1623; Risk levels were set according to the fitting function: OD600≥0.1623 no risk，0.0812＜OD600＜0.1623 low risk，0.0000＜OD600≤0.0812 medium risk，OD600≤0.0000 high risk.