148. The CYP1A1 and COMT genetic polymorphisms and the possible modulation on risk parameters in women who had hypertension during pregnancy

Introduction The immunological alterations during pregnancy may contribute to the onset of preeclampsia (PE) and will affect the future cardiovascular risk (FC-risk) in women with previous PE. Objectives/Hypothesis Although not clear, beyond being involved in the metabolism of estrogens, CYP1A1 and COMT polymorphisms may have a significant impact on immune response. We studied these polymorphisms in the development of PE and FC-risk. Methods We studied a case-control study of 142 pregnant women with normal blood pressure (BP) in pregnancy (NT) and 185 PE; a prospective sub-sample of 138 women of which 90 had PE during pregnancy, 2–16 years ago. The CYP1A1 and COMT polymorphisms were evaluated by PCR-RFLP. The anthropometric, demographic, hemodynamic and biochemical parameters (hepatic function, lipid profile) were determined by conventional methods. Statistical analysis were binary logistic regression and comparison of means, consideringsignificant results for P  Results Women COMT -HL genotype had increased risk for ⩽34 weeks of gestation (WG⩽34), adjusted for age at pregnancy (OR = 2.40, 95% IC [1.04–5.50], P = 0.039]. In the sub-sample, women CYP1A1 -TT genotype or COMT -HL genotype were at increased risk for PE, adjusted for age at pregnancy ( CYP1A1 -TT: OR = 7.30, 95% IC [1.31–40.60, P = 0.023; COMT -HL: OR = 2.85 95% IC [1.08–7.54, P = 0.034). Women who developed hypertension CYP1A1 -TT genotype had higher values of apolipoprotein A (P = 0.064) comparing with CYP1A1 -TC+CC genotypes. Further, in this sub-sample, nitrites values were higher for COMT -HL genotype than COMT -HL+LL genotypes (P = 0.007). Considering only risk genotypes, we found that women currently hypertensive and CYP1A1 -TT, presented systolic(S) BP and diastolic(D) BP higher than NT; and COMT -HL had higher values of SBP, DBP, waist circumference, nitrates and lower HDL (P  Discussion Our results suggested a genetic risk profile associated with CYP1A1 -TT and COMT- HL genotypes for the development of PE and it may modulate the immune adaptation for FC-risk.