Microcalorimetric studies of drug property differences during combination of Rhizoma Coptidis with Fructus Evodiae after separated decoction

Objective To study drug property differences during combination of Rhizoma Coptidis with Fructus Evodiae after separated decoction from biophysics and biochemistry.Methods Rhizoma Coptidis and Fructus Evodiae were decocted with water separately.Different proportions of Rhizoma Coptidis aqueous extract and Fructus Evodiae aqueous extract were combined and heated in an aqueous bath for 1 h at 100℃to get the decoctions of Zuojin Pill,Ganlu Powder,Zhuyu Pill,and Transzuojin Pill.Microcalorimetry was used to obtain the metabolic thermal spectrum curves and biothermo-kinetic parameters of Shigella dysenteriae growth.According to the ancient herbal literatures,the drug property differences during combination of Rhizoma Coptidis with Fructus Evodiae after separated decoction were synthetically evaluated.Results There was a stable drug property difference between separated decoctions.Both the decoction of Zuojin Pill and its similar prescriptions could inhibit the growth and metabolism of the bacteria at different levels.Zuojin Pill had the strongest inhibitory effect,while Transzuojin Pill had the poorest inhibitory effect on S.dysenteriae growth.The sequence of antibacterial activity was.Zuojin PillGanlu PowderZhuyu PillTranszuojin Pill.Conclusion Microcalorimetry can be used to investigate the drug property differences during combination of Rhizoma Coptidis with Fructus Evodiae after separated decoction.