An immunoassay for the simultaneous detection of antigens and No antibodies in a test sample of a physiological fluid.

This invention provides a INMUNOENSARO IN SOLID PHASE FOR THE DETERMINATION OF MEMBERS OF A COUPLE IMMUNE of antigen and / or antibody ONE PAIR OF UNION IN A human physiological fluid, wherein an immunoassay UNICO can simultaneously detect antigen and / or antibody A PAIR OF UNION SINGULAR in a test sample which may contain antigen and / or antibody CIRCULANTE. The immunoassay is characterized by the addition of a quantity DENATIGENO O PAIR OF UNION TO SAMPLE TO BE TESTED BEFORE incubating said sample in the presence of an antibody ABSORBED solid phase binding pair. Preferably, the test sample is also subjected to a reactive lysing before incubation TO RELEASE UNIFORMLY Antigens may be present in the test sample.