РАЗРАБОТКА ПРОМЫШЛЕННОГО СПОСОБА КУЛЬТИВИРОВАНИЯ ПРОДУЦЕНТА РЕКЭМБИ-НАНТНОГО ТКАНЕВОГО АКТИВАТОРА ПЛАЗМИНОГЕ-НА ЧЕЛОВЕКА

An industrial perfusion method for culturing of the CHO 1F8 cell line, a producer of human recombinant tissue plasminogen activator, has been developed. It permits to obtain a product that meets the requirements of the European Pharmacopoeia in respect of specific activity, content of the single-strand form and glycosylation profile. The suggested method makes it possible to obtain 38+3 g rTPA, or 750 doses of drug per cycle in a 100-L bioreactor.