Rubicon PicoPlex-NGS Kits Available for Sequencing Single Cells Using the Illumina Genome Analyzer

Rubicon Genomics has released its PicoPlex-NGS kits to prepare single cells for NGS analysis on the Illumina Genome Analyzer. These kits enable single eukaryotic or prokaryotic cells to be lysed, DNA extracted and amplified, and adapted for paired-end sequencing in a 1-tube, 3-hr, 4-step process. Although the read coverage is poor in a single lane, the reproducibility of the reads allows single cells to be compared for SNP and CNV genotype, mutations. The same amplified samples can be used for PCR and microarray analysis, including genome-wide SNP genotyping, mutation, and copy number analysis. PRC amplification or target enrichment can be used for high accuracy and coverage single cell genomic analysis.