APC is epigenetically down regulated in advance cases of gallbladder cancer

Methods We carried out methylation specific PCR of the modified genomic DNA from GBC and GSD tissues, followed by semi-quantitative and quantitative PCRs (qPCR). We compared the transcript levels of the two exons of APC in GBC and GSD as compared to their adjacent control tissues. We also performed Immunohistochemistry (IHC) on tissue micro array (TMA) of GBC and GSD. The scoring of different tissue cores was done by the expert pathologist and student’s t-test was performed to check the significance.