Two-photon and Multi-color Fluorogenic Bioorthogonal Probes based on Tetrazine-conjugated Naphthalene Fluorophores.
2020
Herein, we report the use of two-photon fluorogenic probes using tetrazine-based bioorthogonal reactions with multi-color emissions that cover nearly all the visible region. New fluorogenic probes were designed based on donor-acceptor type naphthalene structures conjugated with a fluorescence-quenching tetrazine moiety for turn-on properties in one- and two-photon fluorescence. Our fluorescent probes showed a moderate to good turn-on ratio after bioorthogonal inverse electron demand Diels-Alder cycloaddition with trans-cyclooctenol in one- and two- photon fluorescence. We successfully applied our probes to mitochondria- and lysosome-selective bioorthogonal imaging in live cells with one-/two-photon and one-photon microscopy, respectively.
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