Study on the Relationship of Dexamethasone-induced Mouse Thymo cytes Apoptosis Process with ERK1/2 MAPK Pathway and Calcium Ion Concentration

AIM: To study the relationship of thy mo cyte apoptosis with ERK1/2 MAPK pathway and calcium ion concentration, and to f urther discuss possible apoptosis mechanism METHOD:Based on dexamethasone-induced mouse thymocyte apoptosis model,flow cyto metry and transmission electronic microscope analysis were used to determine the model fea sibility, adopted real-time confocal microscope analysis to measure intracellular i changing trend,and utilized SDS-page as well as Western blot anal ysis to ob serve the total and phosphorylated ERK1/2 contentRESULT: T he apoptosis percentage of RPMI1640 control group was (290±062)%, while by 2 5×10 - 6 mol/L DEX treatment, it was (1270±244)% (3 h),(5355±459)% (6 h),P001, r espectively The intracellular fluorescence intensity was also significantly e nhance d No obvious total ERK1/2 content change trend was observed in control group, w hile in DEX group, total ERK1/2 decreased and phosphorylated ERK1/2 was signific antly inhibited following time lapse CONCLUSION: By the stimul atio n of DEX, ERK1/2 pathway was inhibited rapidly,and intracellular i was increased quickly We assumed that they had coherently relations hip