Sequencing and Homology Analysis for the Nucleocapsid Gene of JS/95/03 Strain of Avian Infectious Bronchitis Virus Isolated from Jiangsu, China

Field avian infectious bronchitis virus (IBV) designated as JS/95/03, which was isolated from Jiangsu province of china, was cultivated in chicken embryo. It's single strain RNA was extracted from purified virus and worked as template of reverse transcription polymerase chain reaction (RT PCR), a pair of primer designed according to megalign results of published IBV sequences in Genbank was used to amplify the neucleocapsid gene, the RT PCR product was sequenced directly. Sequence analysis revealed that the sequence of JS/95/03 is most homologized with that of M41 strain.