Physical mapping of differences between the chloroplast DNAs of Beta vulgaris and B. webbiana

A physical map of Beta webbiana (a wild species of the section Pro-cumbentes) chloroplast genome was constructed by localizing the cleavage sites of SmaI, PstI, PvuII, XhoI, and HindIII, and the map was then aligned with the map of sugarbeet (B. vulgaris) chloroplast DNA. This alignment shows 27 restriction-site changes and 11 insertions/deletions, most of which occur in the large single-copy region of the genome. A 0.7-kb long mutation, located within an unidentified open reading frame (ORF2280) in the inverted repeat, was also found.