Hairy Root Induced by Agrobacterium rhizogenes in Soybean

12 soybean materials were infected by Agrobacterium rhizogenes A4,and daidzein content was determined in hairy roots which were induced by inoculating Agrobacterium rhizogenes A4."Hefeng 35" was tested to be the best receptor among the 12 materials.Inoculating by needle on soybean cotyledon node was the best method for inducing hairy roots,the induced rate was 58%.Daidzein content in hairy roots was 2 times of that in seedling cultivated in medium and field.