Synthesis and Antimetastatic Effect of E-salignone

The preparation of novelE-salignone derivatives and their biological evaluation as potential antimetastatic agents is described.The E-salignone amide derivatives were prepared from epiandrosterone and androsterone, and characterized by analytical 1 H NMR, 13 C NMR, and mass spectrometry. The derivatives were evaluated for antimetastatic activity in MDA-MB-231 cells by using a transwell assay. Comparing with the positive control, LY294002, compounds 19b, 19d, and 19e exhibited significant inhibitory effects on the EGF-induced invasion of MB-MDA-231 cells. Moreover, compound 19b also had antimigrationeffectsinwound-healingassay.Compound19bmayrepresentanovelantimetastaticagentfor treating breast cancer. Drug Dev Res 75 : 76-87, 2014. © 2013 Wiley Periodicals, Inc.