Original Article Expression of chemokine receptor CXCR7 and its effects on gastric cancer tissues and cell lines
2016
Gastric cancer (GC), the most common and malignant tumor of the digestive system, exhibits high inva- sive capacity that escapes immune attack. Chemokine receptor CXCR7 plays an important role in the development of cancer. Therefore, the aim of the present study is to investigate the expression of CXCR7 in GC tissues and to evaluate the role of CXCR7 in tumor growth, apoptosis, and invasion of GC cells. The expression status of CXCR7 was detected in 65 primary GC tissues by immunohistochemistry. The correlation of CXCR7 expression with the clinicopathologic parameters and prognostic factors of GC was analyzed. Further, the effects of CXCR7 knock-down by CXCR7-shRNA lentiviral vector on the proliferation, apoptosis, and invasion of GC cells were explored in vitro. MTT assay, FCM analysis, and transwell chamber test were employed. The expression of CXCR7 was significantly higher in GC tissues than in normal tissues. CXCR7 expression was correlated with the degree of differentiation, depth of invasion, and lymph node metastasis. Transfection of MKN-45 cells with CXCR7-shRNA lentiviral vector resulted in a significantly decreased expression of CXCR7 at an mRNA and protein level. Tumor invasiveness was suppressed in vitro by silencing of CXCR7 in MKN-45 cells. However, no obvious suppression in cell proliferative activity and apop- tosis were observed. The expression of CXCR7 could be used as a biomarker to predict prognosis and metastasis of GC. LV-CXCR7-shRNA constructs effectively inhibited the expression of CXCR7 RNA, thus reducing the migration and invasion capacity of human GC cells in vitro.
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