Prevalence of platelet‐specific antibodies and efficacy of crossmatch‐compatible platelet transfusions in refractory patients
2014
SUMMARY
Background
The development of specific antibodies against human leukocyte antigen (HLA) and/or human platelet antigen (HPA) could induce platelet transfusion refractoriness especially in patients receiving multiple platelet transfusions. A retrospective analysis was conducted to evaluate the prevalence of platelet-specific antibodies and the efficacy of crossmatch-compatible platelet transfusions in these recipients.
Study Design and Methods
All enrolled patients were refractory to random single-donor apheresis Platelet (PLT) units. Enzyme-linked immunosorbent assay (ELISA) was used to detect anti-HLA and anti-HPA antibodies in serum. For those patients with antibodies, the PLT crossmatch assays were performed to select the compatible PLTs with a commercial solid-phase adherence kit.
Results
A total of 193 patients were included and 29·02% of which was HLA and/or HPA antibody-positive. There were no significant differences in antibody-positive rates among AML/CML, ALL/CLL, MDS, SAA and ITP groups, but they are statistically significantly higher than other groups (P = 0·0035). Of those antibody-positive patients, there were 41 (73·21%) patients with only HLA antibodies, 11 (19·64%) patients with only HPA antibodies and 4 (7·14%) patients with both HLA and HPA antibodies. A total of 43 random PLT units and 88 crossmatch-compatible PLT units were administered. The mean (±SD) corrected count increment (CCI) was 8700 (±4500) after crossmatch-compatible unit transfusion, significantly higher than 3600 (±2400) for random PLT units (P < 0·001).
Conclusions
HLA and/or HPA alloimmunisation is an important factor to cause refractoriness to platelet transfusions. Crossmatch-compatible platelet transfusion is an effective method in those patients refractory to random platelet transfusions.
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