Use of monoclonal antibodies to protein F of Pseudomonas aeruginosa as opsonins for phagocytosis by macrophages.

Abstract Five protein F-specific monoclonal antibodies were found to opsonize Pseudomonas aeruginosa for complement-independent phagocytosis by unelicited mouse peritoneal macrophages, mouse macrophage cell line P388D1, and human monocyte-derived macrophages. Immunoglobulin G1 antibodies seemed to be a preferred isotype.