Novel imaging of the prostate reveals spontaneous gland contraction and excretory duct quiescence together with different drug effects

Prostate carcinoma and benign prostate hyperplasia (BPH) with associated lower urinary tract symptoms (LUTS) are among the most prevalent and clinically relevant diseases in men. BPH is characterized by an enlargement of prostate tissue associated with increased tone of smooth muscle cells (SMCs) which surround the single glands composing the prostate. Secretions of the glands leave the prostate through local excretory ducts during the emission phase of ejaculation. Pharmacological treatment of BPH suggests different local drug targets based on reduction of prostate smooth muscle tone as the main effect and disturbed ejaculation as a common side effect. This highlights the need for detailed investigation of single prostate glands and ducts. We combined structural and functional imaging techniques—notably, clear lipid-exchanged, acrylamide-hybridized rigid imaging/immunostaining/in situ hybridization–compatible tissue-hydrogel (CLARITY) and time-lapse imaging—and defined glands and ducts as distinct SMC co...