Development and Validation of the Quantification of GML-1 Compound ( N -benzyl- N -methyl-1-phenylpyrrolo[1,2- A ] pyrazine- 3-carbamide) in the Blood Plasma of Rats by HPLC–MS

The technique of quantitative determination of a new compound, possessing anxiolytic activity, GML-1 in the blood plasma of rats is developed and validated. The analysis is performed by HPLC–MS. The method is linear in the range of 50–1000 ng/mL. 83.63% of GML-1 was recovered. The detection limit was 25 ng/mL.