Generation and characterization of human induced pluripotent stem cell lines from a familial Alzheimer's disease PSEN1 A246E patient and a non-demented family member bearing wild-type PSEN1

Abstract The induced pluripotent stem cell (iPSC) lines UOWi002-A and UOWi003-A were reprogrammed from dermal fibroblasts via mRNA transfection. Dermal fibroblasts from a 56 year old female caucasian familial Alzheimer's disease patient carrying A246E mutation in the PSEN1 gene (familial AD3, autopsy confirmed Alzheimer's disease) and a 75 year old female non-demented control from the same family bearing the wild-type PSEN1 A246 genotype were obtained from the Coriell Institute (AG06848 and AG06846, respectively). The generated iPSCs were characterized and pluripotency was confirmed. The PSEN1 genotype was maintained in both iPSC lines. Resource table. Unique stem cell lines identifier UOWi002-A UOWi003-A Alternative names of stem cell lines iPSC 6846.2 (UOWi002-A) iPSC 6848.2 (UOWi003-A) Institution Illawarra Health and Medical Research Institute, University of Wollongong Contact information of distributor Lezanne Ooi, lezanne@uow.edu.au Type of cell lines iPSC Origin Human Cell source Dermal fibroblast Clonal Single clone Method of reprogramming Transgene free Multiline rationale Family members, one demented patient with a familial Alzheimer's disease mutation and one non-demented control Gene modification No Type of modification N/A Associated disease Non-demented control (UOWi002-A) Familial Alzheimer's disease (UOWi003-A) Gene/locus N/A (UOWi002-A) PSEN1 A246E (UOWi003-A) Method of modification N/A Name of transgene or resistance N/A Inducible/constitutive system N/A Date archived/stock date 24/04/2018 Cell line repository/bank N/A Ethical approval HE13/299 University of Wollongong Human Research Ethics Committee