Neuregulin-1 temperature-dependently inhibits the long-term potentiation in the CA1 region in mouse hippocampal slices

Objective To investigate the effect of neurogulin-1(NRG1) on the transmission and plasticity of CA1 synapses in mouse hippocampal slices at different temperatures.Methods Under room temperature(26±1 ℃) or physiologcial temperature(32±1 ℃), field excitatory postsynaptic potential(fEPSP) evoked by extracellular microelectrode recording technique was recorded in the CA1 region in adult mouse hippocampal slices, and the long-term potentiation(LTP) was induced by high frequency stimulation(HFS).The effects of NRG1 on the baseline fEPSP and induction of LTP in CA1 region were observed.Results No significant variation in the slope of fEPSP relative to the baseline fEPSP was observed after perfusion with NRG1 at room temperature or physiological temperature(P0.05).After HFS at the room temperature, the mean slope of fEPSP in the slices perfused with NRG1 was similar to that of the control group(P0.05), but HFS at the physiological temperature resulted in significant reduction in the mean slope of fEPSP in the slices perfused with NRG1(P0.01).Conclusion NRG1 may temperature-dependently inhibit the induction of LTP in the CA1 region in mouse hippocampal slices.