Pt-based cytostatics: Where are they accumulated?

One of the main group of drugs that inhibit cell growth are Pt-based cytostatics – cis-, carbo- and oxali-Pt. They are simple molecules with incorporated Pt and their treatment differs. Our hypothesis is that this various treatment should results in a different distribution of Pt in melanoma tissue. For this purpose, we examined 4 different groups of mice with prostatic melanoma. Three of them were treated by cis-, carbo-, and oxali-Pt, respectively and fourth was not treated any drug. The melanomas were resected from each mice and were cut into thin section form. Distribution of Pt, Cu, Zn was studied using LA-ICP-MS method. The distinct differences are observable not only in the distribution of Pt but also in the amount of Pt that got into tumor tissue. Moreover, distribution of Cu and Zn varies, as well depending on applied cytostatic. The obtained results could be useful in explanation of cytostatics treatment and their mechanism.