The effect of monosialoganglioside on expression of phosphoinositide 3 kinase in hippocampus of APP/PSl double transgenic mice of Alzheimer disease

Objective To study the effect of monosialoganglioside(GM1)on leaming and memory and the expression level of phosphoinositide 3-kinases(PI3K)in hippocampus of APP/PSl double transgenic mice of Alzheimer disease(AD). Methods 20APP/PSl double transgenic mice were randomly divided into AD model group(10),monosialoganglioside treatment group(10)and control group(10). The behavioral scores were investigated by step-down test and water maze test.The expression of PI3K was examined in hippocampus of APP/PSl double transgenic by immunohistochemistry and western blot methods. Results Compared with AD group,the grades of learning and memory of mice in monosialoganglioside treatment group and control group were obviously increased(P<0.05);The mean optical density(MOD)of PI3K positive expression was higher of hippocampus in mice of monosialoganglioside treatment group and control groups than in mice of AD model group detected by immunohistochemistry method and western blot method. Conclusion The improvement of learning and memory abilities in AD mice might be related to the upregulation of PI3K expression in hippocampus caused by GM1.