Method and system for deducing bonding site of oligonucleotide on genome

The invention provides a method and system for deducing a bonding site of oligonucleotide on a genome. According to the method, an index table of thermodynamics of arbitrary 7-mer oligonucleotide is built, a stable bonding sequence of the oligonucleotide to be deduced on the thermodynamics is acquired by using the index table, the position of the bonding sequence is positioned on the genome, thus, the bonding site of the oligonucleotide to be deduced can be efficiently deduced, and a basis is further provided for judging whether the oligonucleotide is high-quality oligonucleotide with regard to a target sequence in the genome. The invention also provides a system for the bonding site of the oligonucleotide on the genome. With the adoption of the above method by the system, the real bonding condition of the oligonucleotide and the target sequence in the genome can be efficiently reflected from the thermodynamics property, and an accurate basis can be provided for judging the quality of the oligonucleotide to be deduced.