Detecção molecular e análise filogenética da sequência parcial do gene da proteína do capsídeo do vírus da faixa das nervuras do morangueiro

The cultivated strawberry, Fragaria x ananassa Duch. (Rosaceae) is a hybrid from the crossing of American species Fragaria chiloensis and Fragaria virginiana, belonging to the Rosaceae family. Strawberry plants have vegetative reproduction and, therefore, it is common the propagation of viruses and other diseases. One of the four most important viruses in strawberry is the Strawberry vein banding virus (SVBV), which is transmitted by aphids in a semi-persistent manner. Molecular techniques are recommended to improve genome knowledge and classification of the SVBV isolates. The coat protein (CP) is a primary determinant of aphid transmissibility and specificity, critically important to infection establishment. In the present work, we sequenced the SVBV coat protein partial gene of a German strain, inoculated in strawberry plants maintained in green house in Brazil for ten years. It was performed phylogenetic analysis comparing to SVBV coat protein sequences from the GenBank database. Aiming to elucidate evolutionary relationships in this species, phylogenetic analysis showed that our SVBV sequence is closely related to USA and Egypt virus strains. These results will contribute to a better elucidation of evolutionary mechanisms and the SVBV-strawberry pathosystem in question.