Flux profiling of photosynthetic carbon metabolism in intact plants

Robert Heise,Stéphanie Arrivault,Marek Szecowka,Takayuki Tohge,Adriano Nunes-Nesi,Mark Stitt,Zoran Nikoloski,Alisdair R. Fernie

Flux profiling of photosynthetic carbon metabolism in intact plants

2014

Robert Heise
Stéphanie Arrivault
Marek Szecowka
Takayuki Tohge
Adriano Nunes-Nesi
Mark Stitt
Zoran Nikoloski
Alisdair R. Fernie

Full characterization of the metabolic state of a biological system involves quantifying the flux of the biochemical reactions. In this protocol, Arabidopsis rosettes are treated with 13CO2, and 13C-labeled metabolites are analyzed by GC- and LC-MS.

Keywords:

Metabolic pathway
Arabidopsis thaliana
Metabolism
Flux
Arabidopsis
Carbon
Photosynthesis
Biology
Biochemistry
metabolic state
photosynthetic carbon metabolism
biochemical reactions

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