Determination of vitamins B group concentrations in Dorema aucheri by HPLC-UV

Solid phase extraction (SPE) was proposed for simultaneous extraction of trace levels of vitamins B1,B2,B3,B5,B6,B7,B9 and B12. SPE method was proposed for determination of vitamins B in Dorema aucheri. High performance liquid chromatography with UV detector (HPLC-UV) was employed to analysis the vitamins. The affecting parameters in chromatographic conditions such as,pH,ratios of the solvents and temperature were studied. Under the optimal conditions,relative standard deviations (RSD) of the analysis less than 11 (n= 3) and detection limit were detected less than 1 ng kg-1 for vitamins. The proposed method was successfully applied for the determination of vitamins B in Dorema aucheri. © 2016,International Journal of Pharmacy and Technology. All rights reserved.