Immunofluorescence Procedure for Developing Enamel Tissues

: Immunofluorescence (IF) labeling is a powerful technique that can provide a wealth of information on structural organization, supramolecular composition, and functional properties of cells and tissues. At the same time, nonspecific staining and false positives can seriously compromise IF studies and lead to confusing or even misleading results. It is particularly true for the extracellular matrix component of forming enamel. Here, we present an optimized IF protocol for developing enamel. Autofluorescence blocking by Sudan Black B (SBB) and establishing of proper isotype controls lead to a significant artifact reduction and improve reliability of the IF data.