Tissue section cutting method for sea water left eye floundre and right eye founder fertilized egg

The present invention relates to microscopic tissue section technology, and establishes one effective tissue section method for the fertilized egg of sea water left eyed founder and right-eyed founder based on the features of the fertilized egg of sea water left eyed founder and right-eyed founder. The fertilized egg is polylecithal pelagic egg with high egg membrane toughness and narrow perivitelline space. The tissue section method includes the following steps: 1. fixing the polylecithal pelagic egg with Bouini»s fixing fluid and optimizing treatment period; 2. pricking the egg membrane with dissecting needle in the vegetative pole direction to peel off the egg membrane; and 3. directly embedded to fix embryo with hot agar. The method is simple and effective, and may be also applied in the tissue section of other sea water fish egg.