OP0308 SENESCENT PROGENITOR CELLS IN THE SKIN OF LUPUS PATIENTS

Background: Systemic lupus erythematosus (SLE) is an autoimmune disease, which is characterized by skin lesions, amongst other symptoms. These lesions (chronic discoid lupus erythematosus (CDLE) and subacute cutaneous lupus erythematosus (SCLE)) feature lymphocytic infiltration close to basal layer of the epidermis (i.e. the location of the epidermal progenitor cells), namely the epidermal dermal junction (EDJ) area. Epidermal progenitor cells maintain the homeostasis of the skin through their proliferation and differentiation into keratinocytes. In fast turnover tissues, like the skin, a population of ‘transient amplifying cells’ (TA cells), additionally facilitates generation of enough daughter cells to maintain skin homeostasis. These cells are located in an upper layer (suprabasal layer) of the epidermis, next to the basal layer. Senescence is an irreversible and locally spreading phenomenon that induces permanent cell cycle arrest. Objectives: To evaluate expression of senescence markers p16 and p21 in the epidermal progenitor and TA niches in patients with SCLE and CDLE. This was compared to a panel of other dermatological conditions with and without infiltration close to EDJ, as disease controls, and to control skin tissue. Methods: Age-matched skin lesions from patients with SCLE (n=12), CDLE (n=8), other conditions with EDJ infiltration (e.g. lichen planus, n=22), and dermatoses without EDJ infiltration (e.g. eczema, n=27), and non-lesion control biopsies (n=3) from SLE patients were employed. p16 and p21 expression in the progenitor niche (basal layer) and TA cell niche (suprabasal layer), and the whole epidermis of skin lesions biopsies were examined by immunohistochemistry. Results: In healthy skin biopsies, 0 ± 0 SEM p16+ cells/mm2 in the progenitor niche and 5 ± 4 SEM p21+ cells/mm2 in TA niche were observed. In skin lesions from patients with CDLE and SCLE, significantly more p16+ cells in the progenitor cell niche (45 ± 14 SEM/mm2) and p21+ cells (182 ± 38 SEM/mm2) in TA niches were detected, compared to control biopsies (p 0.05). Across all dermatoses analyzed, the number of p16+ cells was significantly correlated with the number of p21+ cells, both in the progenitor niche (r=0.45, p Conclusion: In CDLE and SCLE, cutaneous manifestations of SLE, more progenitor and TA cells expressing markers of senescence were detected. This was in common with other dermatological conditions where lymphocytic infiltration is in close proximity to the progenitor and TA cell niche, namely in the EDJ. Increased senescence might infer the collapse of homeostasis in target (local) epidermis, which may influence tissue repair in the lesion. Elimination of senescent cells may therefore represent a viable therapeutic option to encourage timely and complete wound healing, in skin lesions of CDLE and SCLE patients. Acknowledgements: This research was funded by a China Scholarship Council grant (201606220074), Dutch Arthritis Foundation Translational Research Grant (T015-052) and a Dutch Arthritis Foundation Long Term Project Grant (LLP-29). Disclosure of Interests: None declared.