Problems concerned with gametogenesis in Haemosporidiidea, with particular reference to the genus Plasmodium

Although many interesting observations have been made upon gametogenesis in Haemosporidiidea many fundamental problems await solution. Our knowledge of the nuclear changes occurring during the development of the gametes and the fertilization of the female gamete is still incomplete. Little is known of the factors which control gametocyte production, though there is evidence that in some species, i.e. Leucocytozoon simondi and Haemoproteus columbae in the duck, it is influenced by the physiological stage of the host. The type of host and the method of transmission have also been shown to affect the numbers of gametocytes formed in some species. No correlation has been found in Plasmodium , the genus in which this subject has been studied extensively, between acquired immunity and gametocyte formation. Although a broad relationship has been demonstrated in Plasmodium between the density of gametocytes in the blood and the oocyst rate and density assessed in susceptible mosquitoes, hosts heavily infected with apparently ripe gametocytes may prove non-infective to susceptible mosquitoes. In birds heavily infected with P. gallinaceum , serum taken at the peak of parasitaemia has been shown to affect adversely the infectivity of gametocytes to mosquitoes. Pamaquin and primaquine, in small doses, inhibit the development of gametocytes completely, whereas proguanil may not prevent the formation of oocysts though it inhibits their subsequent development. High densities of gametocytes have been observed in patients treated with proguanil or sulphamethazine. An actual in crease in the number of gametocytes produced, as compared with the untreated control strain, was observed in strains of P. gallinaceum in process of developing resistance to sulphadiazine, proguanil or 2:4-diamino-6:7-diisopropylpteridine as a result of treatment with those drugs.