FlimTK: a Python-based toolkit package for fluorescence lifetime imaging microscopy (FLIM) data analysis

Fluorescence Lifetime Imaging Microscopy (FLIM), providing unique quantitative functional information, has gained popularity in various biomedical and molecular biology studies. Here we present an open-source Python package, FlimTK, a toolkit that enables state-of-the-art functions for FLIM image analysis and visualization. It contains comprehensive functionalities for reading FLIM raw files, fluorescence lifetime estimation, heterogeneity analysis, and spatial distribution analysis. FlimTK package is optimized for high performance and ease of use for integration into custom Python-based analysis workflows. FlimTK source code, demo analysis workflows, and tutorial documentation are available for download from GitHub.