Successful PCR amplification of genomic DNA from Cryptosporidium parvum oocysts extracted from a human faecal sample: a rapid and simple method suited for outbreak analysis
2001
A method to extract genomic DNA from oocysts of Cryptosporidium parvum in human faecal material was developed and consisted of a simple alkali wash, freeze/boil technique. This method was simple, quick, sensitives inexpensive and resulted in the production of genomic DNA which was free of PCR inhibitors and as Such was a suitable template for the detection of C. parvum by various PCR amplification targets.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
10
References
10
Citations
NaN
KQI