Assessment of the anti-HBV like property of BEHP – a phthalate derivative and its insilico characterization

Methods Fresh ink glands from Loligo duvauceli were dissected and the ink was subjected to crude solvent extraction. Chromatographic profiles were assessed by TLC and HPTLC. Fractionation of the bio active compounds was done by Silica gel column chromatography and the active fraction was subjected to GC-MS analysis. In vitro analysis of the anti-HBV like activity was assessed by pre incubating the HBV positive serum samples with the active fraction by performing ELISA. In silico virtual screening was analyzed with the latest bio informatics tools and databases.