Association between the single nucleotide polymorphism at locus 1165 of β1-adrenoceptor and infection by Enterovirus A71

Objective To detect the single nucleotide polymorphism (SNP) at locus 1 165 of β1-adrenoceptor (β1-AR) and to investigate the association between the SNP and the infection by enterovirus A71(EV-A71). Methods Polymerase chain reaction (PCR) amplification technique was used to detect the SNP at locus 1 165 of β1-AR between hand, foot and mouth disease (HFMD) and healthy controls by sanger sequencing method . Results There was a G1165C SNP and three kinds of genotypes (GG, GC, CC) in β1-AR gene in the 77 cases of EV-A71 HFMD patients and 66 cases of healthy controls. For HFMD patients, frequencies of GG, GC and CC genotypes of the G1165C locus were 10%, 47% and 43%, respectively, and alleles frequency of G and C were 34% and 66%, respectively. But in healthy children, GG, GC, CC genotype frequencies were 7%, 41% and 52%, respectively, and G and C allele frequencies were 28% and 72% respectively. Chi-square analysis showed that there were no significant differences in distribution of genotypes (χ2=1.154, df=2, P=0.562) and alleles frequency (χ2=1.091, df=2, P=0.296) between the EV-A71-infected group and the healthy control group. Between mild and severe EV-A71-infected group, there were no significant differences in distribution of genotypes (χ2=3.945, df=2, P=0.139) and alleles frequency (χ2=3.763, df=2, P=0.052). Conclusions The 1 165 SNP in the coding region of β1-AR was not associated with EV-A71 infection and its severity. Key words: β1-adrenoceptor; Single nucleotide polymorphism; Enterovirus A71; Hand, foot and mouth disease