Quantitation of camellianin A in HepG2 cells using a high performance liquid chromatography-electrospray ionization tandem mass spectrometric method

Abstract The present study was designed to develop a sensitive and selective high performance liquid chromatography–tandem mass spectrometric method for the determination of Camellianin A in HepG2 cells. The extraction of Camellianin A was achieved using 15% trichloroacetic acid and then separated on a C 18 column interfaced with a triple quadrupole tandem mass spectrometer in multiple reaction monitoring mode. The mobile phase was consisted of methanol–water (0.1% formic acid) (55 : 45, V / V ). The total run time was 5.0 min. The method was linear in the concentration range of 0.25–250.0 ng·mL −1 . The lower limit of quantification was 0.25 ng·mL −1 . The intra- and inter-day relative standard deviations of entire concentration range were less than 9.3%. The proposed HPLC-MS/MS method was successfully applied to detect the intracellular concentration of Camellianin A in HepG2 cells.