Pharmacokinetics of hylotelephin in Beagle dogs

Aim To investigate the pharmacokinetics of hylotelephin in Beagle dogs and obtain the main pharmacokinetic parameters.Methods An HPLC method with UV detection was developed to study the pharmacokinetics of hylotelephin in dogs by joining an internal standard(anthracene).Benzoyl chloride was used to the pre-column derivatization of hylotelephin and methanol-water(64∶36) was used as the mobile phase.According to the 3P97 pharmacokinetic program,the main parameters were calculated.Results The hylotelephin pharmacokinetics conforms to a two-compartment open model after a single iv dose of hylotelephin 10.6 or 21.3 mg·kg~(-1) in Beagle dogs.The parameters of two groups were as follows: T_(1/2)α were 2.3 and 2.1 min,T_(1/2)β were 1.9 and 2.0 h, K_(12) were 0.12 and 0.11 min,K_(21) were 0.17 and 0.21 min, K_(10) were 0.011 and 0.009 4 min,V_c were 0.54 and 0.54 L·kg~(-1),AUC were 1.8 and 4.1 g·min·L~(-1),CL were 0.004 8 and 0.005 6 L·kg~(-1)·min~(-1),MRT were 2.10 and 2.4 h,respectively.Conclusion The pharmacokinetics of hylotelephin after iv administration showed a rapid distribution and elimination process in Beagle dogs and was of first order kinetics.